Surveillance footage showed the moment a reported drone hit an aid vessel heading for Gaza.
The Global Sumud Flotilla said its boat was attacked by a drone at a Tunisian port, the second such strike in two days.
The GSF said all passengers and crew were unharmed.
On Tuesday the group said another of its vessels was struck in Tunisian waters.
The flotilla is an international initiative seeking to break Israel's naval blockade and deliver humanitarian aid to war-torn Gaza using civilian boats supported by delegations from 44 countries.
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